Experimental Approaches & Methods

We examine the effects of chemokines and viral proteins using different methods including traditional biochemical/molecular biology techniques and novel imaging and proteomics approaches, as briefly described below. Primary cultures of rat and human central neurons are used as a model for the study of chemokine receptors naturally expressed by neurons. Alternative models include neuronal human cell lines expressing specific chemokine receptors. In addition, small animal models and post-mortem brain tissue samples from control and HIV-infected individuals are used for the analyses of specific pathways of interest in vivo/ex vivo.

Working with cells

  • Primary Brain Cultures
  • Rat neuronal/glial cultures
  • Cortical, hippocampal, cerebellar cultures
  • Human brain cultures
  • Cortical neurons and glia

Working in vivo/ex vivo

  • Human brain tissue (Immunohistochemistry/Laser Capture Microdissection)
  • Animal models of opiate use
  • MOR k/o animals, gp120 transgenic animals, and SCID mice

Microscopy techniques and molecular biology

  • Live Cells Calcium Imaging
  • Confocal microscopy
  • Multispectral Imaging (MSI)
  • Laser Capture Microdissection (LCM)
  • Cellular & Molecular biology/pharmacology
  • RT-PCR/qPCR
  • Western Blot
  • ELISA
  • Transfection (electroporation and other methods)
  • RNAi